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The effect of plant growth regulators, nutrient strength of MS medium, sucrose concentration, and photosynthetic photon flux (PPF) on the induction and proliferation of embryogenic callus (EC) in Pimpinella brachycarpa was investigated. The optimal MS medium strength and sucrose concentration for induction and proliferation of EC were 1.0X and 30 gㆍL?¹, respectively. Treatment with 2,4-D resulted in more induction of both EC and non-embryogenic callus (NEC) than other plant growth regulators. The percentage of EC induction was the greatest (40%) after treatment with 2.0 ㎎ㆍL?¹ 2,4-D. Treatment with NAA, IAA, and IBA resulted in rooting and/or shooting, which is disadvantageous for establishing a micropropagation system using somatic embryogenesis. EC proliferation rate was the greatest in auxin-free medium. Better EC induction was found in darkness or dim lighting (20 μ㏖ㆍm?²ㆍs?¹ PPF) compared to higher PPFs. EC proliferation was also retarded at higher PPFs and was best at 0 μ㏖ㆍm?²ㆍs?¹ PPF. After inducing EC from a single explant, the EC mass increased 25 times (from 0.05 to 1.30 g) after 4 weeks when nutritional, chemical and physical factors were optimized.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
Literature Cited

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