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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.12
- 수록면
- 1,372 - 1,375 (4page)
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이 논문의 연구 히스토리 (3)
2007
[P5-38] Growth and Fibrinolytic Activity of Bacillus licheniformis Strain Carrying a Fibrinolytic Gene, AprE2 from Bacillus subtilis
Gun-Hee Kwon
,
Gyoung Min Kim
,
Kang Wook Lee
외 4명
한국식품영양과학회 학술대회발표집
2007.10
학술대회자료
[P5-37] Molecular Cloning of AprE Gene from B. subtilis CH3-5 and Its Expression in B. licheniformis ATCC 10716
Gun-Hee Kwon
,
Gyoung Min Kim
,
Kang Wook Lee
외 4명
한국식품영양과학회 학술대회발표집
2007.10
학술대회자료
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초록· 키워드
오류제보하기
The aprE2 gene from Bacillus subtilis CH3-5 was expressed in Bacillus licheniformis ATCC 10716 using a Bacillus-Escherichai coli shuttle vector, pHY300PLK. The fibrinolytic activity of transformant (TF) increased significantly compared to B. licheniformis 10716 control cell. During the 100 hr incubation in Luria-Bertaini broth at 37℃, fibrinolytic activity of B. licheniformis TF increased rapidly at the late growth stage, after 52 hr of incubation, which was confirmed by zymography using a fibrin gel. pHY3-5 was stably maintained in B. licheniformis without tetracycline (Tc) in the media, 60.9% of cells still maintained pHY3-5 after 100 hr of cultivation.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References
참고문헌 (9)
참고문헌 신청
Ageitos JM / 2007 / Purification and characterization of a milk-clotting protease from Bacillus licheniformis strain USC13 / J. Appl. Microbiol 103:2205~2213
Creusot N / 2007 / Hydrolysis of whey protein isolate with Bacillus licheniformis protease: Fractionation and identification of aggregating peptides / J. Agr. Food Chem 55:9241~5250
Schallmey M / 2004 / Developments in the use of Bacillus species for industrial production / Can. J. Microbiol 50:1~17
Peng Y / 2005 / Microbial fibrinolytic enzymes: An overview of source, production, properties, and thrombolytic activity in vivo / Appl. Microbiol. Biot 69:126~132
HWANG, KYUNG-JU / 2007 / Purification and Characterization of a New Fibrinolytic Enzyme of Bacilluslicheniformis KJ-31, Isolated from Korean Traditional Jeot-gal / Journal of Microbiology and Biotechnology 17(9):1469~1476
Creusot N / 2007 / Hydrolysis of whey protein isolate with Bacillus licheniformis protease: Fractionation and identification of aggregating peptides / J. Agr. Food Chem 55:9241~5250
Schallmey M / 2004 / Developments in the use of Bacillus species for industrial production / Can. J. Microbiol 50:1~17
Peng Y / 2005 / Microbial fibrinolytic enzymes: An overview of source, production, properties, and thrombolytic activity in vivo / Appl. Microbiol. Biot 69:126~132
HWANG, KYUNG-JU / 2007 / Purification and Characterization of a New Fibrinolytic Enzyme of Bacilluslicheniformis KJ-31, Isolated from Korean Traditional Jeot-gal / Journal of Microbiology and Biotechnology 17(9):1469~1476
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UCI(KEPA) : I410-ECN-0101-2013-573-000858670