Honey bee is a highly valued resource around the world that contribute approximately 25% of world food productions and provide various functional foods as well as the potential drugs for disease. A erstwhile methods to determine the fate of honey bees are direct way and have a limitation in their usefulness under the some circumstances. This study is to explore the possibility of the infrared thermal photograph technique as a new indirect diagnostic method for survey of survival or death of honey bees colonies. The thermal infrared images are analyzed by FLIR tools and analyzed data used to examine a difference between survival group and death group. This study showed that the difference between the highest and the lowest temperature of the two groups has a range of about 2~4°C on average and the survival group mostly has a higher temperature in comparison with death group. It indicated that infrared thermal photograph technique could be used as an indicator to determine the fate of honey bee colonies indrectly.