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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제6호
- 발행연도
- 2018.1
- 수록면
- 997 - 1,006 (10page)
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초록· 키워드
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As shown during the 2009 pandemic H1N1 (A(H1N1)pdm09) outbreak, egg-based influenza vaccine production technology is insufficient to meet global demands during an influenza pandemic. Therefore, there is a need to adapt cell culture-derived vaccine technology using suspended cell lines for more rapid and larger-scale vaccine production. In this study, we attempted to generate a high-growth influenza vaccine strain in MDCK cells using an A/ Puerto/8/1934 (H1N1) vaccine seed strain. Following 48 serial passages with four rounds of virus plaque purification in MDCK cells, we were able to select several MDCK-adapted plaques that could grow over 108 PFU/ml. Genetic characterization revealed that these viruses mainly had amino acid substitutions in internal genes and exhibited higher polymerase activities. By using a series of Rg viruses, we demonstrated the essential residues of each gene and identified a set of high-growth strains in MDCK cells (PB1D153N, M1A137T, and NS1N176S). In addition, we confirmed that in the context of the high-growth A/PR/8/34 backbone, A/ California/7/2009 (H1N1), A/Perth/16/2009 (H3N2), and A/environment/Korea/deltaW150/ 2006 (H5N1) also showed significantly enhanced growth properties (more than 107 PFU/ml) in both attached- and suspended-MDCK cells compared with each representative virus and the original PR8 vaccine strain. Taken together, this study demonstrates the feasibility of a cell culture-derived approach to produce seed viruses for influenza vaccines that are cheap and can be grown promptly and vigorously as a substitute for egg-based vaccines. Thus, our results suggest that MDCK cell-based vaccine production is a feasible option for producing large-scale vaccines in case of pandemic outbreaks.
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참고문헌 (40)
참고문헌 신청
Garten RJ / 2009 / Antigenic and genetic characteristics of swineorigin 2009 A (H1N1) influenza viruses circulating in humans / Science 325:197~201
Saito T / 2004 / Antigenic alteration of influenza B virus associated with loss of a glycosylation site due to host-cell adaptation / J. Med. Virol. 74:336~343
Grohskopf LA / 2016 / Prevention and control of seasonal influenza with vaccines: recommendations of the advisory committee on immunization practices (ACIP)-United States, 2016-17 influenza season / MMWR Recomm. Rep. 65:1~54
Dawood FS / 2012 / Estimated global mortality associated with the first 12 months of 2009 pandemic influenza A H1N1 virus circulation: a modelling study / Lancet Infect Dis 12:687~695
Larson RC / 2012 / Modeling the effects of H1N1 influenza vaccine distribution in the United States / Value Health 15:158~166
Saito T / 2004 / Antigenic alteration of influenza B virus associated with loss of a glycosylation site due to host-cell adaptation / J. Med. Virol. 74:336~343
Grohskopf LA / 2016 / Prevention and control of seasonal influenza with vaccines: recommendations of the advisory committee on immunization practices (ACIP)-United States, 2016-17 influenza season / MMWR Recomm. Rep. 65:1~54
Dawood FS / 2012 / Estimated global mortality associated with the first 12 months of 2009 pandemic influenza A H1N1 virus circulation: a modelling study / Lancet Infect Dis 12:687~695
Larson RC / 2012 / Modeling the effects of H1N1 influenza vaccine distribution in the United States / Value Health 15:158~166
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