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Cryo-electron microscopy (cryo-EM) is arguably the most powerful tool used in structural biology. It is an important analytical technique that is used for gaining insight into the functional and molecular mechanisms of biomolecules involved in several physiological processes. Cryo-EM can be separated into the following three groups according to the analytical purposes and the features of the biological samples: cryo-electron tomography (cryo-ET), cryo-single-particle reconstruction, and cryo-electron crystallography. Cryotomography is a unique EM technique that is used to study intact biomolecular complexes within their original environments; it can provide mechanistic insights that are challenging for other EM-methods. However, the resolution of reconstructed three-dimensional (3D) models generated by cryo-ET is relatively low, while single-particle reconstruction can reproduce biomolecular structures having near-atomic resolution without the need for crystallization unless the samples are large (>200 kDa) and highly symmetrical. Cryoelectron crystallography is subdivided into the following two categories according to the types of samples: one category that deals with two-dimensional (2D) crystalline arrays and the other category that uses 3D crystals. These two categories of electron-crystallographic techniques use different diffraction data obtained from still diffraction and continuousrotation diffraction. In this paper, we review crystal-based cryo-EM techniques and focus on the recently developed 3D electron-crystallographic technique called microcrystalelectron diffraction.
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참고문헌 (22)
참고문헌 신청
Asano S / 2016 / In situ cryo-electron tomography: A post-reductionist approach to structural biology / J. Mol. Biol 428 : 332 ~ 343
Bendersky L A / 2001 / Electron diffraction using transmission electron microscopy / J. Res. Natl. Inst. Stand. Technol 106 : 997 ~ 1012
Dubochet J / 2012 / Cryo-EM?the first thirty years / J. Microsc 245 : 221 ~ 224
Frank J / 2009 / Single-particle reconstruction of biological macromolecules in electron microscopy?30 years / Q. Rev. Biophys 42 : 139 ~ 158
Frank J / 2016 / Generalized single-particle cryo-EM?a historical perspective / Microscopy (Oxf) 65 : 3 ~ 8
Bendersky L A / 2001 / Electron diffraction using transmission electron microscopy / J. Res. Natl. Inst. Stand. Technol 106 : 997 ~ 1012
Dubochet J / 2012 / Cryo-EM?the first thirty years / J. Microsc 245 : 221 ~ 224
Frank J / 2009 / Single-particle reconstruction of biological macromolecules in electron microscopy?30 years / Q. Rev. Biophys 42 : 139 ~ 158
Frank J / 2016 / Generalized single-particle cryo-EM?a historical perspective / Microscopy (Oxf) 65 : 3 ~ 8
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