지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 278 - 288 (11page)
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초록· 키워드
오류제보하기
In the present investigation, we attempted to design a protocol to develop a hybrid protein with better bioremediation capacity. Using in silico approaches, a Hybrid Open Reading Frame (Hybrid ORF) is developed targeting the genes of microorganisms known for degradation of organophosphates. Out of 21 genes identified through BLAST search, 8 structurally similar genes (opdA, opd, opaA, pte RO, pdeA, parC, mpd and phnE) involved in biodegradation were screened. Gene conservational analysis categorizes these organophosphates degrading 8 genes into 4 super families i.e., Metallo-dependent hydrolases, Lactamase B, MPP and TM_PBP2 superfamily. Hybrid protein structure was modeled using multi-template homology modeling (3S07_A; 99%, 1P9E_A; 98%, 2ZO9_B; 33%, 2DXL_A; 33%) by Schrödinger software suit version 10.4.018. Structural verification of protein models was done using Ramachandran plot, it was showing 96.0% residue in the favored region, which was verified using RAMPAGE. The phosphotriesterase protein was showing the highest structural similarity with hybrid protein having raw score 984. The 5 binding sites of hybrid protein were identified through binding site prediction. The docking study shows that hybrid protein potentially interacts with 10 different organophosphates. The study results indicate that the hybrid protein designed has the capability of degrading a wide range of organophosphate compounds.
목차
등록된 정보가 없습니다.
참고문헌 (43)
참고문헌 신청
Fritsche W / 2005 / Aerobic degradation of recalcitrant organic compounds by microorganisms, Environmental Biotechnology: Concepts and Applications / Wiley-VCH Verlag GmbH & Co :203~227
Kumar C / 2017 / Stability of a mixed microbial population in a biological reactor during long term atrazine degradation under carbon limiting conditions / Int. Biodeterior. Biodegradation 123:311~319
Paul D / 2005 / Accessing microbial diversity for bioremediation and environmental restoration / Trends Biotechnol 23:135~142
Sayler GS / 2000 / Field applications of genetically engineered microorganisms for bioremediation processes / Curr. Opin. Biotechnol 11:286~289
Fujita M / 1991 / Feasibility of wastewater treatment using genetically engineered microorganisms / Water Res 25:979~984
Kumar C / 2017 / Stability of a mixed microbial population in a biological reactor during long term atrazine degradation under carbon limiting conditions / Int. Biodeterior. Biodegradation 123:311~319
Paul D / 2005 / Accessing microbial diversity for bioremediation and environmental restoration / Trends Biotechnol 23:135~142
Sayler GS / 2000 / Field applications of genetically engineered microorganisms for bioremediation processes / Curr. Opin. Biotechnol 11:286~289
Fujita M / 1991 / Feasibility of wastewater treatment using genetically engineered microorganisms / Water Res 25:979~984
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