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학술저널
저자정보
김윤하 (대전대학교 한의과대학 사상체질의학과교실) 박정환 (대전대학교 한의과대학 사상체질의학과교실) 곽진영 (대전대학교 한의과대학 사상체질의학과교실) 박정미 (대전대학교 한의과대학) 안택원 (대전대학교 한의과대학 사상체질의학과교실)
저널정보
사상체질의학회 JSCM : Journal of Sasang constitutional medicine JSCM : Journal of Sasang constitutional medicine 제28권 제2호
발행연도
2016.1
수록면
147 - 162 (16page)

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Objectives The objective of this study is to develop a taeeumin animal-experimental model induced lung fibrosis with Bleomycin and evaluate the effect on obesity in this animal-experimental model.Methods The subjects were divided into 3 groups : normal group, high fat diet(HFD) control group, and HFD group administered with bleomycin(n=10 per group). To develop taeeumin animal-experimental model with reduced respiratory metabolism, 8-week-old C57BL/6 mice were administered with 0.03ml solution of bleomycin 1U/ml dissolved in distilled water, intratracheal(IT), once. Then, the HFD control group and the experimental group were fed with high fat diet for 6 weeks. Airway hyperresponsiveness(AHR) to methacholine was measured at the 1st and 3rd week after bleomycin was administered. Food intake and body weight were measured at regular time weekly. After the final experiment, blood was gathered by cardiac puncture for bloodchemical examination and organs(liver, fatty tissue) were remoed, weighted, and mRNA was analyzed.Results and Conclusions Through the experiment, it was found that Bleomycin induced Taeeumin animal-experimental models have leptin resistace. In the experimental group administered with Bleomycin, fatty acid synthesizing gene expression increased and energy metabolizing gene expression decreased. As mRNA expression of adiponectin decreased, it was found that Taeeuim animal-experimental model is susceptible to metabolic syndrome and cardiovascular diseases.

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