Evaluation of HER-2/neu Overexpression in Gastric Carcinoma using a Tissue Microarray

Rakhshani, Nasser; Kalantari, Elham; Bakhti, Hadi; Sohrabi, Masoud Reza; Mehrazma, Mitra

추천

검색

자료유형: 학술저널

저자정보: Rakhshani, Nasser (Department of Pathology, Firoozgar Hospital, Iran University of Medical Sciences) Kalantari, Elham (Oncopathology Research Center and Department of Pathology, School of Medicine, Iran University of Medical Sciences) Bakhti, Hadi (Department of Pathology, Firoozgar Hospital, Iran University of Medical Sciences) Sohrabi, Masoud Reza (Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences) Mehrazma, Mitra (Oncopathology Research Center and Department of Pathology, School of Medicine, Iran University of Medical Sciences)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제18호

발행연도: 2014.1

수록면: 7,597 - 7,602 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: Amplification and overexpression of human epidermal growth factor receptor 2 (HER2/neu) oncogene has considerable prognostic value in breast and gastric cancers. This study aimed to evaluate the frequency, overexpression pattern, clinical significance, and concordance between the results for protein expression and gene amplification of HER-2/neu in gastric and gastro-esophageal junction carcinomas. Materials and Methods: In this study, 101 gastric tissue samples which were included in tissue microarray were immunohistochemically examined for overexpression of HER2/neu. Chromogenic in situ hybridization (CISH) was used for HER-2/neu amplification. The correlation of HER2/neu amplification with clinicopathological parameters was also assessed. In addition, concordance between CISH and IHC was detected. Results: This study demonstrated a significant difference in the overexpression of HER2/neu in gastric tumors. The overexpression of HER2/neu was significantly higher in intestinal type, poorly differentiated grade, large size ($5cm{\leq}$) and positive nodal involvement tumors (p-value=0.041, 0.015, 0.038 and 0.071, respectively). Also, amplification of HER2/neu according to CISH test, had a significant positive correlation with tumor size and tumor type (p-value=0.018 and 0.058, respectively).Concordance between CISH and IHC was 76.9% in 101 evaluable samples. Conclusions: IHC/CISH differences were attributed to basolateral membranous immunoreactivity of glandular cells resulting in incomplete membranous reactivity and/or a higher rate of tumor heterogeneity in gastric cancers compared to breast cancers. Therefore, this can be a potential marker for targeted therapy of malignant gastric tumors.

#Chromogenic in situ hybridization #gastric cancer #HER2 #immunohistochemistry #tissue microarray

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요