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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 442 - 447 (6page)
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The non-viral delivery of genes into macrophages, known as hard-to-transfect cells, is a challenge. In this study, the microporation of a CpG-free and small plasmid (pCGfd-GFP) showed high transfection efficiency, sustainable transgene expression, and good cell viability in the transfections of Raw 264.7 and primary bone marrow-derived macrophages. The non-viral method using the pCGfd vector encoding anti-EGFR single-chain Fv fused with Fc (scFv-Fc) generated the macrophages secreting anti-EGFR scFv-Fc. These macrophages effectively phagocytized tumor cells expressing EGFR through the antibody-dependent mechanism, as was proved by experiments using EGFR-knockout tumor cells. Finally, peri-tumoral injections of anti-EGFR scFv-Fc-secreting macrophages were shown to inhibit tumor growth in the xenograft mouse model.
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등록된 정보가 없습니다.
참고문헌 (31)
참고문헌 신청
Leslie M / 2018 / New cancer-fighting cells enter trials / Science 361:1056~1057
Overdijk MB / 2015 / Antibody-mediated phagocytosis contributes to the anti-tumor activity of the therapeutic antibody daratumumab in lymphoma and multiple myeloma / MAbs 7:311~321
Chao MP / 2010 / Anti-CD47antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma / Cell 142:699~713
Shi Y / 2015 / Trastuzumab triggers phagocytic killing of high HER2 cancer cells in vitro and in vivo by interaction with Fcgamma receptors on macrophages / J Immunol 194:4379~4386
Kurdi AT / 2018 / Antibody-Dependent Cellular Phagocytosis by Macrophages is a Novel Mechanism of Action of Elotuzumab / Mol Cancer Ther 17:1454~1463
Overdijk MB / 2015 / Antibody-mediated phagocytosis contributes to the anti-tumor activity of the therapeutic antibody daratumumab in lymphoma and multiple myeloma / MAbs 7:311~321
Chao MP / 2010 / Anti-CD47antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma / Cell 142:699~713
Shi Y / 2015 / Trastuzumab triggers phagocytic killing of high HER2 cancer cells in vitro and in vivo by interaction with Fcgamma receptors on macrophages / J Immunol 194:4379~4386
Kurdi AT / 2018 / Antibody-Dependent Cellular Phagocytosis by Macrophages is a Novel Mechanism of Action of Elotuzumab / Mol Cancer Ther 17:1454~1463
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