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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 275 - 285 (11page)
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초록· 키워드
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In this study, for better understanding of patient-derived xenograft (PDX) generation, angiogenic characteristics during PDX cancerous tissue generation was investigated with different initial cell seeding conditions in the hydrogel. We monitored the angiogenic changes during the formation of in vivo cancer cell line xenografts induced by endothelial cells. Our in vivo cancer tissue formation system was designed with the assistance of tissue engineering technology to mimic patient-derived xenograft formation. Endothelial cells and MIA PaCa-2 pancreatic carcinoma cells were encapsulated in fibrin gel at different mixing configurations and subcutaneously implanted into nude mice. To investigate the effect of the initial cancerous cell distribution in the fibrin gel, MIA PaCa-2 cells were encapsulated as a homogeneous cell distribution or as a cell aggregate, with endothelial cells homogeneously distributed in the fibrin gel. Histological observation of the explanted tissues after different implantation periods revealed three different stages: isolated vascular tubes, leaky blood vessels, and mature cancerous tissue formation. The in vivo engineered cancerous tissues had leaky blood vessels with low expression of the vascular tight junction marker CD31. Under our experimental conditions, complex cancer-like tissue formation was most successful when tumorous cells and endothelial cells were homogeneously mixed in the fibrin gel. The present study implies that tumorous xenograft tissue formation can be achieved with a low number of initial cells and that effective vascularization conditions can be attained with a limited volume of patient-derived cancer tissue. Endothelial cellassisted vascularization can be a potent choice for the effective development of vascularized cancerous tissues for studying patient-derived xenografts, cancer angiogenesis, cancer metastasis, and anticancer drugs.
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참고문헌 (45)
참고문헌 신청
Bianco P / 2001 / Stem cells in tissue engineering / Nature 414:118~121
Langer R / 1993 / Tissue engineering / Science 260:920~926
Karam JP / 2012 / Combining adult stem cells and polymeric devices for tissue engineering in infarcted myocardium / Biomaterials 33:5683~5695
Zhang K / 2017 / Heparin/collagen encapsulating nerve growth factor multilayers coated aligned PLLA nanofibrous scaffolds for nerve tissue engineering / J Biomed Mater Res A 105:1900~1910
Hoganson DM / 2014 / Lung tissue engineering / Front Biosci (Landmark Ed) 19:1227~1239
Langer R / 1993 / Tissue engineering / Science 260:920~926
Karam JP / 2012 / Combining adult stem cells and polymeric devices for tissue engineering in infarcted myocardium / Biomaterials 33:5683~5695
Zhang K / 2017 / Heparin/collagen encapsulating nerve growth factor multilayers coated aligned PLLA nanofibrous scaffolds for nerve tissue engineering / J Biomed Mater Res A 105:1900~1910
Hoganson DM / 2014 / Lung tissue engineering / Front Biosci (Landmark Ed) 19:1227~1239
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