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논문 기본 정보

자료유형
학술저널
저자정보
Ding Shi (University of Chinese Academy of Sciences) Xiao-xia Xi (University of Chinese Academy of Sciences)
저널정보
연세대학교 의과대학 Yonsei Medical Journal Yonsei Medical Journal 제62권 제11호
발행연도
2021.11
수록면
1,005 - 1,015 (11page)
DOI
10.3349/ymj.2021.62.11.1005

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Purpose: This study aimed to investigate the mechanistic downregulation of mucin 6 (MUC6) and its influence on the progression of gastric cancer (GC). Materials and Methods: The expression of MUC6 was examined in 40 GC patients. The methylation status of the MUC6 promoter region was investigated using GC cell lines and GC tissue specimens by immunohistochemistry and/or quantitative polymerase chain reaction (qPCR). MUC6 was knocked down in the gastric epithelial cells (GES-1) cell and overexpressed in the SGC7901 cell. The effects of MUC6 knockdown and overexpression on cell migration and invasion were examined using Transwell assays. The effects of demethylation and methylation on MUC6 expression were examined by western blot, qPCR, or double luciferase reporter assays. Results: The expression of MUC6 in GC with lymph node metastasis and poor pathological stage was significantly lower than that in GC without lymph node metastasis and good pathological stage, respectively. While cell migration and invasion were significantly decreased after overexpression of MUC6, these abilities significantly increased after the knockdown of MUC6. The methylation levels of MUC6 in GC tissues and GC cell lines were significantly higher than those in para-cancerous tissues and normal GES. Promoter methylation could significantly reduce the binding of related transcription factors to the MUC6 promoter. The expression of MUC6 increased with the concentration and time of action of demethylation drugs. Conclusion: Expression of MUC6 was regulated by promotor methylation. This methylation of the MUC6 promoter may lead to significant downregulation of MUC6 in GC and promote the progression of GC.
#MUC6 #methylation #gastric cancer

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참고문헌 (28)

참고문헌 신청
Pinto-de-Sousa J / 2004 / MUC5B expression in gastric carcinoma : relationship with clinico-pathological parameters and with expression of mucins MUC1, MUC2, MUC5AC and MUC6 / Virchows Arch 444:224~230 google schola Senapati S / 2008 / The MUC gene family : their role in the diagnosis and prognosis of gastric cancer / Histol Histopathol 23:1541~1552 google schola Jia Y / 2010 / A comprehensive analysis of common genetic variation in MUC1, MUC5AC, MUC6 genes and risk of stomach cancer / Cancer Causes Control 21:313~321 google schola Nakayama J / 2014 / Dual roles of gastric gland mucin-specific O-glycans in prevention of gastric cancer / Acta Histochem Cytochem 47:1~9 google schola Babu SD / 2006 / Expression profile of mucins(MUC2, MUC5AC and MUC6)in Helicobacter pylori infected pre-neoplastic and neoplastic human gastric epithelium / Mol Cancer 5:10 google schola

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