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논문 기본 정보

자료유형
학술저널
저자정보
Su-Jin Park (Korea Basic Science Institute) Hee-Jin Yoo (Korea Basic Science Institute) Duck-Hyun Kim (Korea Basic Science Institute) Ji-Won Park (Korea Basic Science Institute) Eunji Jeon (Korea Basic Science Institute) Abhik Mojumdar (Korea Basic Science Institute) Kun Cho (Korea Basic Science Institute)
저널정보
한국질량분석학회 Mass Spectrometry Letters Mass Spectrometry Letters Vol.15 No.1
발행연도
2024.3
수록면
49 - 53 (5page)

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초록· 키워드

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Ceramide is a lipid in which sphingoid bases and fatty acids are linked by amide bonds. As a marker of skin disease in the human stratum corneum, its disease-causing and therapeutic effects have been partially confirmed, and it is therefore an important element in commercially available cosmetic formulations. However, structural diversity caused by differences in the chain length, number, and location of hydroxyl groups makes quality control difficult. In this study, a method was established to separate different ceramide species using reversed-phase LC-MS/MS and thus enable qualitative evaluation. Separation of four standards was achieved within a short retention time, and the accuracy and sensitivity of the method were demonstrated by the low limit of detection (LOD) calculated based on the calibration curve showing linearity, with R2 > 0.994. After verification of reproducibility and reliability through intra- and inter-day analyses, the efficiency of the method was confirmed through analysis of commercial cosmetic raw materials.

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Abstract
Introduction
Materials and methods
Results and discussion
Conclusions
References

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