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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 105 - 115 (11page)
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초록· 키워드
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Background: Ginsenosides with less sugar moieties may exhibit the better adsorptive capacity and more pharmacological activities.
Methods: An efficient method for the separation of four minor saponins, including gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, from Panax notoginseng leaves (PNL) was established using biotransformation, macroporous resins, and subsequent preparative high-performance liquid chromatography.
Results: The dried PNL powder was immersed in the distilled water at 50℃ for 30 min for converting the major saponins, ginsenosides Rb1, Rc, Rb2, and Rb3, to minor saponins, gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, respectively, by the enzymes present in PNL. The adsorption characteristics of these minor saponins on five types of macroporous resins, D-101, DA-201, DM-301, X-5, and S-8, were evaluated and compared. Among them, D-101 was selected due to the best adsorption and desorption properties. Under the optimized conditions, the fraction containing the four target saponins was separated by D-101 resin. Subsequently, the target minor saponins were individually separated and purified by preparative high-performance liquid chromatography with a reversed-phase column.
Conclusion: Our study provides a simple and efficient method for the preparation of these four minor saponins from PNL, which will be potential for industrial applications.
Methods: An efficient method for the separation of four minor saponins, including gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, from Panax notoginseng leaves (PNL) was established using biotransformation, macroporous resins, and subsequent preparative high-performance liquid chromatography.
Results: The dried PNL powder was immersed in the distilled water at 50℃ for 30 min for converting the major saponins, ginsenosides Rb1, Rc, Rb2, and Rb3, to minor saponins, gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, respectively, by the enzymes present in PNL. The adsorption characteristics of these minor saponins on five types of macroporous resins, D-101, DA-201, DM-301, X-5, and S-8, were evaluated and compared. Among them, D-101 was selected due to the best adsorption and desorption properties. Under the optimized conditions, the fraction containing the four target saponins was separated by D-101 resin. Subsequently, the target minor saponins were individually separated and purified by preparative high-performance liquid chromatography with a reversed-phase column.
Conclusion: Our study provides a simple and efficient method for the preparation of these four minor saponins from PNL, which will be potential for industrial applications.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (25)
참고문헌 신청
Ma WG / 1999 / Saponins from the roots of Panax notoginseng / Phytochemistry 52:1133~1139
Qi LW / 2010 / American ginseng : potential structure-function relationship in cancer chemoprevention / Biochem Pharmacol 80:947~954
Wan J / 2006 / Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD / J Pharm Biomed Anal 41:1596~1601
Wan JB / 2012 / Chemical investigation of saponins in different parts of Panax notoginseng by pressurized liquid extraction and liquid chromatography-electrospray ionization-tandem mass spectrometry / Molecules 17:5836~5853
Xu YX / 2005 / Inhibitory influence of ginsenoside Rb3 on activation of strychnine-sensitive glycine receptors in hippocampal neurons of rat / Brain Res 1037:99~106
Qi LW / 2010 / American ginseng : potential structure-function relationship in cancer chemoprevention / Biochem Pharmacol 80:947~954
Wan J / 2006 / Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD / J Pharm Biomed Anal 41:1596~1601
Wan JB / 2012 / Chemical investigation of saponins in different parts of Panax notoginseng by pressurized liquid extraction and liquid chromatography-electrospray ionization-tandem mass spectrometry / Molecules 17:5836~5853
Xu YX / 2005 / Inhibitory influence of ginsenoside Rb3 on activation of strychnine-sensitive glycine receptors in hippocampal neurons of rat / Brain Res 1037:99~106
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UCI(KEPA) : I410-ECN-0101-2019-524-000425227