지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.2
- 수록면
- 19 - 25 (7page)
- DOI
- 10.7783/KJMCS.2018.26.1.19
이용수
초록· 키워드
오류제보하기
Background: Inula japonica Thunb. is a plant belonging to the family compositae. Inulae flos (flower of I. britannica var. chinensis Regal.) is the dried flower of I. japonica Thunb. and contains various flavonoids (patulitrin, nepitrin and kaempferol), which have been utilized in traditional oriental medicine to treat nausea, phlegm, and coughs. However, ethanol extract of I. britannica (IJE) has not been previously studied for its use in cancer treatment, and its effects on oxidative stress, or inflammation. Thus, the present study investigated the anti-oxidant, anti-inflammatory, and anti-colorectal cancer effects of IJE using RAW264.7 and HCT-116 cells, which are human colorectal cancer cell line.
Methods and Results: IJE contained flavonoids (80.95 ± 5.3 ㎎/g) and polyphenols (310.53 ± 10.6 ㎎/g). Moreover, it reduced lipopolysaccharide (LPS)-induced nitric oxide (NO) production and H₂O₂-induced oxidative stress by decreasing reactive oxygen species (ROS) levels. Additionally, the 500 ㎍/㎖ IJE treatment increased caspase-3 activity and apoptotic cell death in HCT-116 cells.
Conclusions: These results demonstrate that the anti-cancer effect of IJE against human colorectal cancer cells involves caspase-3 activation and apoptotic cell death. IJE also inhibited LPS-induced NO production, and H2O2-induced oxidative stress in RAW264.7 cells. However, further studies are required to explore how IJE treatment regulates signal transduction in NO and ROS production.
Methods and Results: IJE contained flavonoids (80.95 ± 5.3 ㎎/g) and polyphenols (310.53 ± 10.6 ㎎/g). Moreover, it reduced lipopolysaccharide (LPS)-induced nitric oxide (NO) production and H₂O₂-induced oxidative stress by decreasing reactive oxygen species (ROS) levels. Additionally, the 500 ㎍/㎖ IJE treatment increased caspase-3 activity and apoptotic cell death in HCT-116 cells.
Conclusions: These results demonstrate that the anti-cancer effect of IJE against human colorectal cancer cells involves caspase-3 activation and apoptotic cell death. IJE also inhibited LPS-induced NO production, and H2O2-induced oxidative stress in RAW264.7 cells. However, further studies are required to explore how IJE treatment regulates signal transduction in NO and ROS production.
목차
ABSTRACT
서언
재료 및 방법
결과 및 고찰
REFERENCES
참고문헌 (20)
참고문헌 신청
Blois MS / 1958 / Antioxidant determinations by the use of a stable free radical / Nature 181:1199~1200
차미란 / 2006 / 선복화 (Inula japonica)추출물의 암세포주에 대한 In Vitro 세포독성 / 생약학회지 37(3):130~135
Fellegrini N / 1999 / Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay / Methods in Enzymology 299:379~389
Folin O / 1912 / On phosphotungstic-phosphomolybdic compounds as color reagents / Journal of Biological Chemistry 12:239~243
한미라 / 2013 / 선복화, 감국, 홍화 에탄올 추출물의 주름개선 및항산화 효과 / 대한미용학회지 9(4):29~37
차미란 / 2006 / 선복화 (Inula japonica)추출물의 암세포주에 대한 In Vitro 세포독성 / 생약학회지 37(3):130~135
Fellegrini N / 1999 / Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay / Methods in Enzymology 299:379~389
Folin O / 1912 / On phosphotungstic-phosphomolybdic compounds as color reagents / Journal of Biological Chemistry 12:239~243
한미라 / 2013 / 선복화, 감국, 홍화 에탄올 추출물의 주름개선 및항산화 효과 / 대한미용학회지 9(4):29~37
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