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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 1,315 - 1,321 (7page)
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초록· 키워드
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Bacteriocin is ribosomally synthesized by bacteria and inhibits closely related species. In this study we aimed at isolating lactic acid bacteria producing bacteriocin presenting anti-staphylococcal activity. A Lactococcus lactis strain was isolated from kimchi for the purpose and identified by 16S rRNA gene sequencing. As preliminary tests, optimal culture conditions, stabilities against heat, solvents, and enzymes treatments, and type of action (bacteriostatic or bactericidal) of the bacteriocin were investigated. The optimal culture conditions for production of the bacteriocin were MRS broth medium and $25^{\circ}C$ and $30^{\circ}C$ culture temperatures. The bacteriocin was acidic and the activity was abolished by a protease treatment. Its stability was maintained at $100^{\circ}C$ for 15 min and under treatments of various organic solvents such as methanol, ethanol, acetone, acetonitrile, and chloroform. Finally, the bacteriocin showed bactericidal action against Staphylococcus aureus where 200 AU/mL of the bacteriocin decreased the viable cell count (CFU/mL) of S. aureus by 2.5 log scale, compared with a control (no bacteriocin added) after 4-h incubation.
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참고문헌 (23)
참고문헌 신청
Azad MAK / 2018 / Probiotic species in the modulation of gut microbiota: An overview / Biomed Res Int 2018:9478630
Chikindas ML / 2018 / Functions and emerging applications of bacteriocins / Curr Opin Biotechnol 49:23~28
정동민 / 2011 / Rapid Concentration of Some Bacteriocin-like Compounds Using an Organic Solvent / Food Science and Biotechnology 20(5):1457~1459
Daeschel MA / 1992 / Food biopreservatives of microbial origin / CRC Press :57~80
Dicks LMT / 2018 / A review: The fate of bacteriocins in the human gastro-intestinal tract:Do they cross the gut-blood barrier? / Front Microbiol 9:2297
Chikindas ML / 2018 / Functions and emerging applications of bacteriocins / Curr Opin Biotechnol 49:23~28
정동민 / 2011 / Rapid Concentration of Some Bacteriocin-like Compounds Using an Organic Solvent / Food Science and Biotechnology 20(5):1457~1459
Daeschel MA / 1992 / Food biopreservatives of microbial origin / CRC Press :57~80
Dicks LMT / 2018 / A review: The fate of bacteriocins in the human gastro-intestinal tract:Do they cross the gut-blood barrier? / Front Microbiol 9:2297
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