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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.5
- 수록면
- 489 - 495 (7page)
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초록· 키워드
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Background: Although ginsenosides and saponins in Korea red ginseng (KRG) shows various pharmacological roles, their roles in the inflammatory response are little known. This study investigated the anti-inflammatory role of ginsenosides identified from KRG saponin fraction (RGSF) and the potential mechanism in macrophages.
Methods: The ginsenoside composition of RGSF was identified by high-performance liquid chromatography (HPLC) analysis. An anti-inflammatory effect of RGSF and its mechanisms were studied using nitric oxide (NO) and prostaglandin E₂ (PGE₂) production assays, mRNA expression analyses of inflammatory genes and cytokines, luciferase reporter gene assays of transcription factors, andWestern blot analyses of inflammatory signaling pathways using the lipopolysaccharide (LPS)-treated RAW264.7 cells.
Results: HPLC analysis identified the types and amounts of various panaxadiol ginsenosides in RGSF. RGSF reduced the generation of inflammatory molecules and mRNA levels of inflammatory enzymes and cytokines in LPS-treated RAW264.7 cells. Additionally, RGSF inhibited the signaling pathways of NF-kB and AP-1 by suppressing both transcriptional factors and signaling molecules in LPS-treated RAW264.7 cells.
Conclusion: RGSF contains ginsenosides that have anti-inflammatory action via restraining the NF-kB and AP-1 signaling pathways in macrophages during inflammatory responses.
Methods: The ginsenoside composition of RGSF was identified by high-performance liquid chromatography (HPLC) analysis. An anti-inflammatory effect of RGSF and its mechanisms were studied using nitric oxide (NO) and prostaglandin E₂ (PGE₂) production assays, mRNA expression analyses of inflammatory genes and cytokines, luciferase reporter gene assays of transcription factors, andWestern blot analyses of inflammatory signaling pathways using the lipopolysaccharide (LPS)-treated RAW264.7 cells.
Results: HPLC analysis identified the types and amounts of various panaxadiol ginsenosides in RGSF. RGSF reduced the generation of inflammatory molecules and mRNA levels of inflammatory enzymes and cytokines in LPS-treated RAW264.7 cells. Additionally, RGSF inhibited the signaling pathways of NF-kB and AP-1 by suppressing both transcriptional factors and signaling molecules in LPS-treated RAW264.7 cells.
Conclusion: RGSF contains ginsenosides that have anti-inflammatory action via restraining the NF-kB and AP-1 signaling pathways in macrophages during inflammatory responses.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
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UCI(KEPA) : I410-ECN-0101-2022-524-001328857