RNA-seq analysis reveals the role of a small GTP-binding protein,Rab7, in regulating clathrin-mediated endocytosis and salinity-stress resistance in peanut

Jiongming Sui; Guan Li; Guanxu Chen; Chunmei Zhao; Xiangyuan Kong; Xiaomin Hou; Lixian Qiao; Jingshan Wang

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자료유형: 학술저널

저자정보: Jiongming Sui (Key Laboratory of Qingdao Major Crop Ger) Guan Li (Key Laboratory of Qingdao Major Crop Ger) Guanxu Chen (Key Laboratory of Qingdao Major Crop Ger) Chunmei Zhao (Key Laboratory of Qingdao Major Crop Ger) Xiangyuan Kong (Key Laboratory of Qingdao Major Crop Ger) Xiaomin Hou (Key Laboratory of Qingdao Major Crop Ger) Lixian Qiao (Key Laboratory of Qingdao Major Crop Ger) Jingshan Wang (Key Laboratory of Qingdao Major Crop Ger)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제11권 제1호

발행연도: 2017.2

수록면: 43 - 52 (10page)

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Small GTP-binding proteins, Rab7, are known to be responsive to abiotic stresses in plants, but the molecular mechanism is poorly understood. To investigate how AhRab7 increases resistance to salinity stress in peanut, this study compared a transgenic genotype (S5) that overexpressed the AhRab7 gene and that had high salinity resistance with a non-transgenic genotype (S7) that had low salinity resistance. Digital gene expression (DGE) sequencing was performed with leaves of S5 and S7 before and after salinity-stress treatment. In total, 2697 differentially expressed genes (DEGs) were identified between S5 and S7, and KEGG enrichment analyses showed that the DEGs are involved pathways including endocytosis, lysosome, hormone signaling, phosphatidylinositol signaling, calcium, and others. Among them, 164 were differentially regulated after salinity-stress treatment. Of 164 DEGs, 110 were responsive to salinity stress in S5 and/or S7. The 110 DEGs included genes that encode the following kinds of transcription factors and proteins known to be involved in resistance to salinity stress: WRKY, NAC, MYM-type zinc finger, late embryogenesis abundant proteins, lipid transfer protein, 1-cys peroxiredoxin, aquaporin, oleosin, and others. AhRab7 gene might mediate signaling pathways including phosphatidylinositol, calcium, abscisic acid, etc., and then regulate the expression of transcription factors and downstream genes for ROS scavenging in peanut. The results of this study will be useful for further investigations of the mechanism underlying the role of the AhRab7 gene in resistance to salinity stress in peanut.

#Peanut (Arachis hypogaea) · Rab7 · Overexpression · Salinity stress · Endocytosis

참고문헌 (25)

참고문헌 신청

Agarwal PK / 2008 / Constitutive overexpression of a stress-inducible small GTPbinding protein PgRab7 from Pennisetum glaucum enhances abiotic stress tolerance in transgenic tobacco / Plant Cell Rep 27:105~115

Bolte S / 2000 / Characterization of a small GTP-binding protein of the rab5 family in Mesembryanthemum crystallinum with increased level of expression during early salt stress / Plant Mol Biol 42:923~936

Boucrot E / 2010 / Roles of AP-2in clathrin-mediated endocytosis / PLoS One 5:e10597

Brighouse A / 2010 / Rab protein evolution and the history of the eukaryotic endomembrane system / Cell Mol Life Sci 67:3449~3465

Croizet-Berger K / 2002 / The endocytic catalysts, Rab5a and Rab7, are tandem regulators of thyroid hormone production / Proc Natl Acad Sci USA 99:8277~8282

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