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학술저널
저자정보
Lee, Ji-Hoon (Department of Bioenvironmental Chemistry, Chonbuk National University) Lee, Bong-Joo (Groundwater Department, Korea Institute of Geoscience and Mineral Resources) Yun, Uk (Groundwater Department, Korea Institute of Geoscience and Mineral Resources) Koh, Dong-Chan (Groundwater Department, Korea Institute of Geoscience and Mineral Resources) Kim, Soo Jin (Department of Bioenvironmental Chemistry, Chonbuk National University) Han, Dukki (Faculty of Biotechnology, Jeju National University) Unno, Tatsuya (Faculty of Biotechnology, Jeju National University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제62권 제1호
발행연도
2019.1
수록면
51 - 56 (6page)

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Biofilms facilitate communication among microorganisms for nutrients and protect them from predators and harmful chemicals such as antibiotics and detergents. Biofilms can also act as cores for the development of clogs in many agricultural irrigation systems and in porous media. In this study, we deployed glass units at a depth of 20 m below the ground surface in the groundwater-surface water mixing zone, and retrieved them after 4 months to investigate the potential colonization of indigenous microbial community and possible mineral-microbe assemblages. We observed the periodic formation of microbial colonies by fluorescence dye staining and microscopy, and analyzed the composition of the microbial community in both the mineral-microbe aggregates and groundwater, by next generation sequencing of the 16S rRNA gene amplicons using MiSeq platform. During the course of incubation, we observed an increase in both the mineral-microbe aggregates and content of extracellular polymeric substances. Interestingly, the microbial community from the aggregates featured a high abundance of iron redox-related microorganisms such as Geobacter sp., Comamonadaceae sp., and Burkholderiales incertae sedis. Therefore, these microorganisms can potentially produce iron-minerals within the sediment-microbe-associated aggregates, and induce biofilm formation within the groundwater borehole and porous media.

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